LncRNA HOTAIR regulates anoikis-resistance capacity and spheroid formation of ovarian cancer cells by recruiting EZH2 and influencing H3K27 methylation

This study aims to investigate the role of long non-coding RNA (lncRNA) HOX transcript antisense (HOTAIR) in regulation anoikis resistance ovarian cancer cells, a prerequisite for metastasis and chemoresistance cells. Ovarian SKOV3 cells were cultured an ultra-low attachment system establish model. The relationship between cellular capability HOTAIR expression level was studied by flow cytometry RT-PCR. ability spheroid formation, migration, invasion suspended assessed following knockdown expression. EZH2, H3K27me3, representative targets anoikis-related biomarkers also detected. An increase duration suspension culture time rendered anoikis-resistant with significantly lower apoptotic rate compared adherent increased significantly, while that did not. Following small interfering (siRNA)-mediated expression, abilities resistance, decreased Knockdown levels reduced forming tumor continuous cultures. Moreover, EZH2 correlated thus regulating miR-193a DOK2 via introducing H3K27me3. Western blot analysis markers showed N-cadherin, ZEB1, TWIST1 downregulated inhibition HOTAIR, E-cadherin ErbB3 upregulated. In conclusion, enhances recruiting influencing H3K27 methylation may contribute invasion,